Facilities
Environmental And Bioanalytical Chemistry Unit (FQM-141)
Laboratory Menu
LABORATORIES
The Environmental and Bioanalytical Chemistry Group is located at the Faculty of Experimental Sciences (University of Huelva):
- Laboratory for simple preparison (Ex-P1-N5-01)- 40 m2
- Laboratory for Metabolomics (Ex-P1-N5-08)- 60 m2
- Laboratory for Trace Metal Analysis (Ex-P1-N5-07)- 60 m2
- Laboratory for Chemical Spaciation and Metallomics (Ex-P2-N5-07)- 60 m2
INSTRUMENTATION
Chromatography and Electrophoresis
- Gas chromatograph coupled to ion trap mass spectrometer TRACE GC ULTRA ITQ 900 (Thermo Fisher Scientific)
- Gas chromatograph coupled to electron capture detector (GC-µECD, model 6890, Agilent Technologies)
- Liquid chromatograph with UV-vis spectrophotometric detector (HPLC-UV, Thermo Fisher Scientific, Accela PDA 80 Hz Detector)
- Liquid chromatograph with molecular fluorescence detector (HPLC-FLD, Agilent Technologies)
- Nano and micro-HPLC (Agilent Technologies)
- Gas chromatograph coupled to flame ionization detector (GC-FID) (Varian)
- Gas chromatograph coupled to pulsed flame photometric detector (GC-PFPD) (Varian)
- Capillary electrophoresis with UV detector (Beckman)
- Bidimensional electrophoresis system.
Atomic mass spectrometry
- Inductively coupled plasma coupled to cuadrupole reaction cell (ICP-MS) Thermo Xseries 2 (con celda de colisión/reacción). Sistema de introducción automática de muestras
- Inductively coupled plasma with triple quadrupole (ICP-QQQ-MS) Agilent 8800 (Agilent Technologies). Autosampler and bio-inert HPLC model 1260 Infinity.
- Laser ablation system NW100181
Organic mass spectrometry
- Ultra-high performance liquid chromatography coupled to cuadrupole time of flight mass spectrometer (UHPLC-QTOF) Agilent 6550 iFunnel (Agilent Technologies)
- Ultra-high performance liquid chromatography model 1290 Infinity II and ion mobility (Agilent GS 6560, Agilent Technologies) coupled to cuadrupole time of flight mass spectrometer.
- Matrix assisted laser desorption ionization coupled to time of flight mass spectrometer (MALDI-TOF-MS) Voyager Applied Biosystem
Sample preparation
- Pressurized liquid extraction
- Focalized microwave
- Microwave system for simple mineralization with low and high-volume reactors (CEM Mars 240/50)
- Crio-homogeneizers for tissues.
- Tissue Lyser (Fisher Scientific 1.0 DISRUPTOR GENIE 230 v)
- Ultrasound bath
- Ultrasound probe (SONOPLUS mini20)
Other instrumentation and material
- Termostatized UV-vis spectrophotometer with peltier system (Fisher Scientific, Evolution 201)
- 2 centrifuges, one of them termostatized (Eppendorf AG Centrifuge 5804 R
- Speed vaccum (SpeedVac SPD 111V)
- Rotary evaporators
- Filtration systems
- Ultrapure water purification system (Milli-Q Direct 8)
- 2 Stoves
- 4 Fridges
- 2 Freezers -20 º C
- 3 Freezers a -80ºC
PROJECTS FOR FACILITIES ACQUISITION
- Instrumental platform for lipidomics, proteomics and metallomics.
- Instrumental platform for metabolomics.
- Instrumental platform for metallomics, proteomics and environmental pollutants related to health, environment and food analysis based on UHPLC-ICP-QQQ-MS.